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تعداد نتایج: 1469

Subcellular Fractionation

A Practical Approach

By John M. Graham, David Rickwood
Publisher: Oxford University Press, 1997
Pages: 360
Language: en
ISBN_13:9780199634941
ISBN_10:0199634947
Subject: Medical

$Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.$

جزئیات و درخواست

By Paul R. Pryor
2015
Language: en
ISBN_10:1621820424
ISBN_13:9781621820420
Subject: Cell fractionation

Molecular Biology of the Cell

جزئیات و درخواست

2002
Language: en
ISBN_10:0815332181
ISBN_13:9780815332183
Subject: Cells

Nuclear Structure and Function

جزئیات و درخواست

By Miguel Berrios
Publisher: Academic Press, 1998
Pages: 659
Language: en
ISBN_13:9780125641555
ISBN_10:0125641559
Subject: Medical

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

جزئیات و درخواست

Publisher: Academic Press, 2013-10-30
Pages: 408
Language: en
ISBN_13:9780124200944
ISBN_10:012420094X
Subject: Science

Methods in Plant Cell Biology

جزئیات و درخواست

By David W. Galbraith, Hans J. Bohnert, Don P. Bourque
Publisher: Academic Press, 1995
Pages: 605
Language: en
ISBN_13:9780125641517
ISBN_10:0125641516
Subject: Medical

Bacterial Protein Secretion Systems

Methods and Protocols

جزئیات و درخواست

By Laure Journet, Eric Cascales
Publisher: Humana Press, 2017-07-01
Pages: 528
Language: en
ISBN_10:1493970313
ISBN_13:9781493970315
Subject: Science

Subcellular Fractionation

A Practical Approach

$Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.$

جزئیات و درخواست

By J. M. Graham, D. Rickwood
Publisher: IRL Press, 1997-01-30
Pages: 362
Language: en
ISBN_13:9780191591617
ISBN_10:0191591610
Subject: Science

Techniques in Microbial Ecology

جزئیات و درخواست

By Robert S. Burlage
1998
Pages: 484
Language: en
ISBN_10:0195092236
ISBN_13:9780195092233
Subject: Medical

Eukaryotic Cell Function and Growth

Regulation by Intracellular Cyclic Nucleotides

جزئیات و درخواست

By Jacques E. Dumont, Barry L. Brown, Nicholas J. Marshall
Publisher: Springer, 1976-08
Pages: 872
Language: en
OTHER:UCAL:B4300016
Subject: Medical

Cell Biology Protocols

$As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.$

جزئیات و درخواست

By J. Robin Harris, John M. Graham, David Rickwood
Publisher: Wiley, 2012-01-18
Pages: 418
Language: en
ISBN_10:0470033460
ISBN_13:9780470033463
Subject: Science

Discovering Cell Mechanisms

The Creation of Modern Cell Biology

جزئیات و درخواست

By William Bechtel
Publisher: Cambridge University Press, 2006
Pages: 346
Language: en
ISBN_10:052181247X
ISBN_13:9780521812474
Subject: Philosophy

Analytical Techniques in Biochemistry and Molecular Biology

جزئیات و درخواست

By Rajan Katoch
Publisher: Springer Science & Business Media, 2011-07-19
Pages: 449
Language: en
ISBN_13:9781441997852
ISBN_10:1441997857
Subject: Science

Subcellular Fractionation

A Practical Approach

جزئیات و درخواست

By M. Graham
1997
Pages: 360
Language: en
ISBN_10:6613977241
ISBN_13:9786613977243

Plant Biochemistry

جزئیات و درخواست

By P. M. Dey, J. B. Harborne
Publisher: Elsevier, 1997-02-03
Pages: 571
Language: en
ISBN_13:9780080525723
ISBN_10:0080525725
Subject: Science

Adipose Tissue Protocols

$Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.$

جزئیات و درخواست

By Gérard Ailhaud
Publisher: Springer Science & Business Media, 2008-02-03
Pages: 332
Language: en
ISBN_13:9781592592319
ISBN_10:1592592317
Subject: Science

Visions of Cell Biology

Reflections Inspired by Cowdry's "General Cytology"

$Although modern cell biology is often considered to have arisen following World War II in tandem with certain technological and methodological advances—in particular, the electron microscope and cell fractionation—its origins actually date to the 1830s and the development of cytology, the scientific study of cells. By 1924, with the publication of Edmund Vincent Cowdry’s General Cytology, the discipline had stretched beyond the bounds of purely microscopic observation to include the chemical, physical, and genetic analysis of cells. Inspired by Cowdry’s classic, watershed work, this book collects contributions from cell biologists, historians, and philosophers of science to explore the history and current status of cell biology. Despite extraordinary advances in describing both the structure and function of cells, cell biology tends to be overshadowed by molecular biology, a field that developed contemporaneously. This book remedies that unjust disparity through an investigation of cell biology’s evolution and its role in pushing forward the boundaries of biological understanding. Contributors show that modern concepts of cell organization, mechanistic explanations, epigenetics, molecular thinking, and even computational approaches all can be placed on the continuum of cell studies from cytology to cell biology and beyond. The first book in the series Convening Science: Discovery at the Marine Biological Laboratory, Visions of Cell Biology sheds new light on a century of cellular discovery.$

جزئیات و درخواست

By Karl S. Matlin, Jane Maienschein, Manfred D. Laubichler
Publisher: University of Chicago Press, 2018-01-19
Pages: 379
Language: en
ISBN_13:9780226520650
ISBN_10:022652065X
Subject: Science

Bioprocessing for Cell-Based Therapies

جزئیات و درخواست

By Che J. Connon
Publisher: John Wiley & Sons, 2017-02-06
Pages: 276
Language: en
ISBN_13:9781118743416
ISBN_10:1118743415
Subject: Science

Radiobiology for the Radiologist

جزئیات و درخواست

By Eric J. Hall, Amato J. Giaccia
Publisher: Lippincott Williams & Wilkins, 2012-03-28
Pages: 908
Language: en
ISBN_13:9781451154184
ISBN_10:1451154186
Subject: Medical

Cellular Radiobiology

جزئیات و درخواست

By T. Alper
Publisher: Cambridge University Press, 1979-08-16
Pages: 340
Language: en
ISBN_10:052122411X
ISBN_13:9780521224116
Subject: Science

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