The Central Flyway has been recognized as a collective North- South migratory pathway centered on the North American Great Plains for nearly a century, but it has never been analyzed as the species that most closely follow it, or the major stopping points used by those species on their journeys between their northern breeding and southern wintering grounds. A total of 114 U.S. and 21 Canadian localities of special importance to birds migrating within the Central Flyway are identified and described in detail. Judging from available regional, state and local information, nearly 400 species of 50 avian families regularly use the Central Flyway during their migrations. Nearly 90 Central Flyway species have wintering areas partly extending variably far into the Neotropic zoogeographic realm, and at least 50 of these winter entirely within the Neotropic realm. A few of these species undertake some of the longest known migrations of all birds, in excess of 8,000 miles in each direction. Seven maps, 49 figures and over 100 literature citations are included.